elisa quantikine human total mmp 3 immunoassay kit  (R&D Systems)

Journal: Neoplasia (New York, N.Y.)

Article Title: Pancreatic cancer cells infiltrate nerves through TGFbeta1-driven perineural epithelial-to-mesenchymal-like transdifferentiation.

doi: 10.1016/j.neo.2025.101126

Figure Lengend Snippet: Fig. 2. TGFbeta1-secretion and impact of tumor cell supernatants on the expression of alphaSMA, N-Cadherin and cytokeratin 19 (CK 19) in human perineural epithelial cells (HPNEC). A. Assessment of TGFbeta1-secretion in the supernatants of Panc1, SU86.86 and T3M4 via ELISA B. Representative western blot images of the alphaSMA con tent in perineural cells after conditioned media (CM) treatment. Graph repre senting the relative alphaSMA content in HPNEC after CM treatment with the supernatants of the different cancer cell lines. C. Representative western blot images of the N-Cadherin content in HPNEC after conditioned media (CM) treatment. Densitometry graph representing the relative N-Cadherin content of HPNEC after CM treatment with the supernatants of the different cancer cell lines. D. Representative western blot images of the CK19 content in HPNEC after conditioned media (CM) treatment. Densitometry graph representing the relative CK19 content of HPNEC after CM treatment with the supernatants of the different cancer cell lines.

Article Snippet: The levels of MMP-3 and MMP-9 in the supernatants of HPNEC were quantified using Proteintech-KE00160 Human Total MMP-3 ELISA Kit and Proteintech-KE00164 Human MMP-9 ELISA Kit, respectively, according to the manufacturer’s instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Marine Drugs

Article Title: Chondroitin Sulfate Nanovectorized by LC-PUFAs Nanocarriers Extracted from Salmon ( Salmo salar ) by Green Process with Decreased Inflammatory Marker Expression in Interleukin-1β-Stimulated Primary Human Chondrocytes In Vitro Culture

doi: 10.3390/md22120571

Figure Lengend Snippet: The effect of nanoliposomes/CS exposure on IL-1β-stimulated MMPs. Human chondrocytes were stimulated or not with 1 ng/mL IL-1β and exposed to nanoliposomes (250 µg/mL) or nanoliposomes and CS extracts (250 µg/mL+ 25 µg/mL). For ( A , B , E ), total RNA was extracted after 24 h stimulation then reverse transcribed into cDNA and analyzed by RT-PCR. The relative abundance of MMP1, MMP3, and MMP13 mRNAs was normalized to RP29 mRNA. Comparison was performed using the ΔCt method with the fold value of reference = 1. The results shown are the mean ± SD of at least four individual experiments (*: p < 0.01 vs. control; #: p < 0.01 vs. IL-1β). For ( C , D , F ) levels of MMP1, 3, and 13 after 48 h of stimulation (*: p < 0.01 vs. control; #: p < 0.01 vs. IL-1β).

Article Snippet: After stimulations, supernatants were collected and centrifuged 5 min at 600 g and analyses were performed according to the kit manufacturer’s instructions for MMP1, MMP3, and MMP13 levels (DuoSet ® ELISA, R&D systems, Abingdon, UK—Human Total MMP1, Human Total MMP3, and Human Pro-MMP13) and PGE2 levels (Prostaglandin E2 Parameter Assay Kit, Bio-Techne, Abingdon, UK).

Techniques: Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Comparison, Control

Journal: Marine Drugs

Article Title: Chondroitin Sulfate Nanovectorized by LC-PUFAs Nanocarriers Extracted from Salmon ( Salmo salar ) by Green Process with Decreased Inflammatory Marker Expression in Interleukin-1β-Stimulated Primary Human Chondrocytes In Vitro Culture

doi: 10.3390/md22120571

Figure Lengend Snippet: Sequences of specific primers for RT-PCR analyses.

Article Snippet: After stimulations, supernatants were collected and centrifuged 5 min at 600 g and analyses were performed according to the kit manufacturer’s instructions for MMP1, MMP3, and MMP13 levels (DuoSet ® ELISA, R&D systems, Abingdon, UK—Human Total MMP1, Human Total MMP3, and Human Pro-MMP13) and PGE2 levels (Prostaglandin E2 Parameter Assay Kit, Bio-Techne, Abingdon, UK).

Techniques: